Beta Catenin Monoclonal antibody
Beta Catenin Monoclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IP, IHC, IF, CoIP,ELISA
Conjugate
Unconjugated
CloneNo.
1B8A1
Cat no : 66379-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, pig brain tissue, MCF-7 cells, HEK-293 cells, HepG2 cells, LNCaP cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, rat colon tissue, mouse colon tissue, human colon tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells, human skin cancer tissue, human liver cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 21 publications below |
| IHC | See 2 publications below |
| IF | See 6 publications below |
| CoIP | See 2 publications below |
Product Information
66379-1-Ig targets Beta Catenin in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, pig, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Calculated molecular weight | 781 aa, 86 kDa |
| Observed molecular weight | 92 kDa |
| GenBank accession number | NM_001904 |
| Gene symbol | CTNNB1 |
| Gene ID (NCBI) | 1499 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
| IHC protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
| IF protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
| IP protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res RUNX1 promotes tumour metastasis by activating the Wnt/β-catenin signalling pathway and EMT in colorectal cancer. | ||
Oxid Med Cell Longev Gender Difference on the Effect of Omega-3 Polyunsaturated Fatty Acids on Acetaminophen-Induced Acute Liver Failure. | ||
Cell Death Dis Overexpressed P75CUX1 promotes EMT in glioma infiltration by activating β-catenin. | ||
Pediatr Pulmonol Effect of electro-acupuncture at ST 36 on maternal food restriction-induced lung phenotype in rat offspring. | ||
FEBS Open Bio SOX6 suppresses the development of lung adenocarcinoma by regulating expression of p53, p21CIPI , cyclin D1 and β-catenin. |
