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beta-Catenin Antibody 5 Publications

Mouse Monoclonal| Catalog number: 66379-1-Ig |CloneNo.: 1B8A1

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, COIP, ELISA
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Images See all

beta-Catenin Antibody WB HeLa cells 66379-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (beta-Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

beta-Catenin Antibody IP mouse brain tissue 66379-1-Ig

IP result of anti-beta-Catenin(IP:66379-1-Ig, 4ug; Detection:66379-1-Ig 1:2000) with mouse brain tissue lysate 4400 ug

beta-Catenin Antibody IHC human liver cancer tissue 66379-1-Ig

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:5000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

beta-Catenin Antibody IHC human liver cancer tissue 66379-1-Ig

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:5000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

beta-Catenin Antibody IF MCF-7 cells 66379-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
HeLa cells, pig brain tissue, MCF-7 cells, HEK-293 cells, HepG2 cells, LNCaP cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human liver cancer tissue, human skin cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells, human skin cancer tissue, human liver cancer tissue
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:2500-1:10000
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight:
781aa,86 kDa
Observed molecular weight:
92 kDa
GenBank accession number:
NM_001904
Gene ID (NCBI):
1499
Gene symbol
CTNNB1
Synonyms
b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286

Background

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)


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