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Beta Catenin Monoclonal antibody
Beta Catenin Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 66379-1-Ig
Validation Data Gallery
|Positive WB detected in
|A431 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, rabbit brain tissue
|Positive IP detected in
|mouse brain tissue
|Positive IHC detected in
|mouse colon tissue, human skin cancer tissue, human liver cancer tissue, rat colon tissue, human breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|MCF-7 cells, human liver cancer tissue, human skin cancer tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:10000-1:40000
|IF : 1:750-1:3000
|Flow Cytometry (FC)
|FC : 0.50 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66379-1-Ig targets Beta Catenin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
|human, mouse, rat, pig, rabbit
|human, mouse, rat, sheep, pig
|Host / Isotype
|Mouse / IgG1
|catenin (cadherin-associated protein), beta 1, 88kDa
|Calculated molecular weight
|781 aa, 86 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
|Product Specific Protocols
|WB protocol for Beta Catenin antibody 66379-1-Ig
|IHC protocol for Beta Catenin antibody 66379-1-Ig
|IF protocol for Beta Catenin antibody 66379-1-Ig
|IP protocol for Beta Catenin antibody 66379-1-Ig
|FC protocol for Beta Catenin antibody 66379-1-Ig
|Click here to view our Standard Protocols
Acta Pharm Sin B
Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.
Research (Wash D C)
The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling
Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering
Riboflavin deficiency leads to irreversible cellular changes in the RPE and disrupts retinal function through alterations in cellular metabolic homeostasis.
CRISPR/Cas9 library screening uncovered methylated PKP2 as a critical driver of lung cancer radioresistance by stabilizing β-catenin.
Clin Transl Med
Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness.