Tested Applications
| Positive WB detected in | A431 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, rabbit brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse colon tissue, human breast cancer tissue, human colon tissue, human ovary cancer tissue, human skin cancer tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue, human skin cancer tissue |
| Positive IF/ICC detected in | MCF-7 cells, human liver cancer tissue, human skin cancer tissue |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:10000-1:40000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 89 publications below |
| IHC | See 12 publications below |
| IF | See 34 publications below |
| IP | See 1 publications below |
| CoIP | See 7 publications below |
Product Information
66379-1-Ig targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, sheep |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Calculated Molecular Weight | 781 aa, 86 kDa |
| Observed Molecular Weight | 92 kDa |
| GenBank Accession Number | NM_001904 |
| Gene Symbol | Beta Catenin |
| Gene ID (NCBI) | 1499 |
| ENSEMBL Gene ID | ENSG00000168036 |
| RRID | AB_2857358 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P35222 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
| IHC protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
| IF protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
| IP protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
| FC protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2. | ||
J Exp Clin Cancer Res Membrane RRM2-positive cells represent a malignant population with cancer stem cell features in intrahepatic cholangiocarcinoma
| ||
Research (Wash D C) The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling | ||
Acta Biomater Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering | ||
Redox Biol Riboflavin deficiency leads to irreversible cellular changes in the RPE and disrupts retinal function through alterations in cellular metabolic homeostasis. | ||
Oncogene CRISPR/Cas9 library screening uncovered methylated PKP2 as a critical driver of lung cancer radioresistance by stabilizing β-catenin.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Bruna (Verified Customer) (05-30-2024) | Great antibody! In our lab, it was used for Western blot and immunofluorescence detection. In both cases, the results were excellent.
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