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Beta Catenin Monoclonal antibody
Beta Catenin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig and More (1)
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
1B8A1
Cat no : 66379-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, rabbit brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse colon tissue, human skin cancer tissue, human liver cancer tissue, rat colon tissue, human breast cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human colon cancer tissue, human skin cancer tissue |
Positive IF/ICC detected in | MCF-7 cells, human liver cancer tissue, human skin cancer tissue |
Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:10000-1:40000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 4 publications below |
WB | See 76 publications below |
IHC | See 10 publications below |
IF | See 30 publications below |
IP | See 1 publications below |
CoIP | See 7 publications below |
Product Information
66379-1-Ig targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, pig, sheep |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
Calculated Molecular Weight | 781 aa, 86 kDa |
Observed Molecular Weight | 92 kDa |
GenBank Accession Number | NM_001904 |
Gene Symbol | Beta Catenin |
Gene ID (NCBI) | 1499 |
RRID | AB_2857358 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
Protocols
Product Specific Protocols | |
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WB protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
IHC protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
IF protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
IP protocol for Beta Catenin antibody 66379-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Pharm Sin B Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2. | ||
J Exp Clin Cancer Res Membrane RRM2-positive cells represent a malignant population with cancer stem cell features in intrahepatic cholangiocarcinoma
| ||
Research (Wash D C) The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling | ||
Acta Biomater Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering | ||
Redox Biol Riboflavin deficiency leads to irreversible cellular changes in the RPE and disrupts retinal function through alterations in cellular metabolic homeostasis. | ||
Oncogene CRISPR/Cas9 library screening uncovered methylated PKP2 as a critical driver of lung cancer radioresistance by stabilizing β-catenin.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Bruna (Verified Customer) (05-30-2024) | Great antibody! In our lab, it was used for Western blot and immunofluorescence detection. In both cases, the results were excellent.
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