CoraLite®488-conjugated Beta Catenin Monoclonal antibody

Beta Catenin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1B8A1

Cat no : CL488-66379

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β-Catenin



Tested Applications

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66379-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:100
Sample-dependent, check data in validation data gallery

Product Information

CL488-66379 targets Beta Catenin in IF applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
GenBank accession numberNM_001904
Gene symbol CTNNB1
Gene ID (NCBI) 1499
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222) This antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).

Protocols

Product Specific Protocols
IF protocol for CL488 Beta Catenin antibody CL488-66379Download protocol
Standard Protocols
Click here to view our Standard Protocols