CoraLite® Plus 488-conjugated Beta Catenin Monoclonal antibody
Beta Catenin Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B8A1
Cat no : CL488-66379
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-66379 targets Beta Catenin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
Calculated molecular weight | 781 aa, 86 kDa |
GenBank accession number | NM_001904 |
Gene symbol | CTNNB1 |
Gene ID (NCBI) | 1499 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222) This antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Beta Catenin antibody CL488-66379 | Download protocol |
FC protocol for CL Plus 488 Beta Catenin antibody CL488-66379 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |