CXorf21 Recombinant monoclonal antibody

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• CXorf21 Recombinant monoclonal antibody (88004-1-RR)

• 

  WB analysis using 88004-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88004-1-RR (CXorf21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 88004-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88004-1-RR (CXorf21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 88004-1-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 88004-1-RR (CXorf21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of THP-1 using 88004-1-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CXorf21 antibody (88004-1-RR, Clone: 260203H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CXorf21 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 88004-1-RR

Clone No.260203H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

TASL, TLR adapter interacting with SLC15A4 on the lysosome

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, THP-1 cells, J774A.1 cells
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88004-1-RR targets CXorf21 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag32773

  Product name: Recombinant human CXorf21 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-301 aa of BC020611

  Sequence: MLSEGYLSGLEYWNDIHWSCASYNEQVAGEKEEETNSVATLSYSSVDETQVRSLYVSCKSSGKFISSVHSRESQHSRSQRVTVLQTNPNPVFESPNLAAVEICRDASRETYLVPSSCKSICKNYNDLQIAGGQVMAINSVTTDFPSESSFEYGPLLKSSEIPLPMEDSISTQPSDFPQKPIQRYSSYWRITSIKEKSSLQMQNPISNAVLNEYLEQKVVELYKQYIMDTVFHDSSPTQILASELIMTSVDQISLQVSREKNLETSKARDIVFSRLLQLMSTEITEISTPSLHISQYSNVNP

• Full Name: chromosome X open reading frame 21
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC020611
• Gene Symbol: CXorf21
• Gene ID (NCBI): 80231
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HAI6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXorf21 (Chromosome X open reading frame 21) is also named as TASL and TLR adapter interacting with SLC15A4 on the lysosome. CXorf21 is an innate immune adapter that mediates the recruitment and activation of IRF5 downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PMID:32433612). CXorf21 plays a role in the regulation of endolysosomal pH in immune cells such as B-cells, dendritic cells and monocytes (PMID: 31001245). CXorf21 is a mediator of the X-chromosome gene dose-dependent increased risk of SLE in females (PMID: 31695690).