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Calretinin Antibody 2 Publications

Mouse Monoclonal| Catalog number: 66496-1-Ig |CloneNo.: 2D7A9

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Calretinin Antibody WB mouse brain tissue 66496-1-Ig

mouse brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours

Calretinin Antibody IHC human brain tissue 66496-1-Ig

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig( Calretinin antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Calretinin Antibody IHC human brain tissue 66496-1-Ig

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig( Calretinin antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
Human, mouse, pig, rat
Positive WB detected in:
mouse brain tissue, pig brain tissue, U-251 cells, rat brain tissue
Positive IHC detected in:
human brain tissue, human cerebellum tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:20000-1:80000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Calretinin fusion protein Ag2924
Full name:
calbindin 2
Calculated molecular weight:
29 kDa
Observed molecular weight:
29 kDa
GenBank accession number:
BC015484
Gene ID (NCBI):
794
Gene symbol
CALB2
Synonyms
29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

Background

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.


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