Calretinin Monoclonal antibody
Calretinin Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, pig, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2D7A9
Cat no : 66496-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, pig brain tissue, U-251 cells, rat brain tissue |
Positive IHC detected in | human brain tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:80000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
IF | See 3 publications below |
Product Information
66496-1-Ig targets Calretinin in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.
Tested Reactivity | Human, mouse, pig, rat |
Cited Reactivity | mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Calretinin fusion protein Ag2924 |
Full Name | calbindin 2 |
Calculated molecular weight | 29 kDa |
Observed molecular weight | 29 kDa |
GenBank accession number | BC015484 |
Gene symbol | CALB2 |
Gene ID (NCBI) | 794 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.
Protocols
Product Specific Protocols | |
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WB protocol for Calretinin antibody 66496-1-Ig | Download protocol |
IHC protocol for Calretinin antibody 66496-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis Differential effects of N-acetylcysteine on retinal degeneration in two mouse models of normal tension glaucoma. | ||
Invest Ophthalmol Vis Sci Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma. | ||
Cell Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. |