Calretinin Monoclonal antibody

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  WB analysis using 66496-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of rat cerebellum using 66496-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 66496-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66496-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66496-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66496-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 66496-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 66496-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human appendicitis using 66496-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human appendicitis using 66496-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of SH-SY5Y using 66496-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Calretinin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 66496-1-Ig

Clone No.2D7A9

Publications(11)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

CALB2, calbindin 2, CAL2, CAB29, 2D7A9

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Tested Applications

• Positive WB detected in: pig brain tissue, mouse brain tissue, rat brain tissue, U2OS cells, rat cerebellum tissue, U-251 cells, mouse cerebellum tissue, rabbit brain tissue
• Positive IHC detected in: human appendicitis tissue, human brain tissue, human cerebellum tissue, human colon tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human appendicitis tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Product Information

66496-1-Ig targets Calretinin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calretinin fusion protein Ag2924
• Full Name: calbindin 2
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC015484
• Gene Symbol: Calretinin
• Gene ID (NCBI): 794
• RRID: AB_2868480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P22676
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
mouse	WB,IF	Acta Neuropathol Commun; TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.; Authors - Tuancheng Feng
mouse	WB,IF	Neurobiol Dis; Sex specific correlation between GABAergic disruption in the dorsal hippocampus and flurothyl seizure susceptibility after neonatal hypoxic-ischemic brain injury.; Authors - Charles R Lechner
mouse	IF	Neurobiol Dis; Cytohesin-2 mediates group I metabotropic glutamate receptor-dependent mechanical allodynia through the activation of ADP ribosylation factor 6 in the spinal cord.; Authors - Akiko Ito
human	WB	J Cancer; A Prognostic Model of Angiogenesis and Neutrophil Extracellular Traps Related Genes Manipulating Tumor Microenvironment in Colon Cancer; Authors - Dongsheng Zhang
	IHC	Mol Brain; A new AAV tool for highly preferentially targeting hippocampal CA2; Authors - Siqi Peng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (09-05-2024)

Calretinin (green) worked magnificently on my human brain FFPE tissue marking some neurons (NeuN in red)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Calretinin Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:66496-1-Ig)