Calretinin Monoclonal antibody

Calretinin Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2D7A9

Cat no : 66496-1-Ig

Synonyms

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

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Validation Data Gallery

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  WB analysis of mouse brain using 66496-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, pig brain tissue, U-251 cells, rat brain tissue
• Positive IHC detected in: human brain tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:80000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 3 publications below

Product Information

66496-1-Ig targets Calretinin in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

• Tested Reactivity: Human, mouse, pig, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calretinin fusion protein Ag2924
• Full Name: calbindin 2
• Calculated molecular weight: 29 kDa
• Observed molecular weight: 29 kDa
• GenBank accession number: BC015484
• Gene symbol: CALB2
• Gene ID (NCBI): 794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Publications

Species	Application	Title
mouse	IF	Cell Death Dis; Differential effects of N-acetylcysteine on retinal degeneration in two mouse models of normal tension glaucoma.; Authors - Hiroki Sano
mouse	IF	Invest Ophthalmol Vis Sci; Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma.; Authors - Kei Akaiwa
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou