Calretinin Monoclonal antibody

Calretinin Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D7A9

Cat no : 66496-1-Ig

Synonyms

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR



Tested Applications

Positive WB detected inmouse brain tissue, pig brain tissue, U-251 cells, rat brain tissue
Positive IHC detected inhuman brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:80000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 3 publications below

Product Information

66496-1-Ig targets Calretinin in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calretinin fusion protein Ag2924
Full Name calbindin 2
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC015484
Gene symbol CALB2
Gene ID (NCBI) 794
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Protocols

Product Specific Protocols
WB protocol for Calretinin antibody 66496-1-IgDownload protocol
IHC protocol for Calretinin antibody 66496-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Death Dis

Differential effects of N-acetylcysteine on retinal degeneration in two mouse models of normal tension glaucoma.

Authors - Hiroki Sano
mouseIF

Invest Ophthalmol Vis Sci

Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma.

Authors - Kei Akaiwa
mouseIF

Cell

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Authors - Haibo Zhou