CoraLite®555-conjugated Calretinin Monoclonal antibody

Calretinin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2D7A9

Cat no : CL555-66496

Synonyms

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

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Validation Data Gallery

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  IF Staining of SH-SY5Y using CL555-66496

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®555 Calretinin antibody (CL555-66496, Clone: 2D7A9 ) at dilution of 1:200.

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  IF Staining of human appendicitis using CL555-66496

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 Calretinin antibody (CL555-66496, Clone: 2D7A9 ) at dilution of 1:200.

• 

  IF Staining of human appendicitis using CL555-66496

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 Calretinin antibody (CL555-66496, Clone: 2D7A9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: SH-SY5Y cells, human appendicitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66496 targets Calretinin in IF applications and shows reactivity with Human, mouse, pig, rat samples.

• Tested Reactivity: Human, mouse, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calretinin fusion protein Ag2924
• Full Name: calbindin 2
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC015484
• Gene Symbol: CALB2
• Gene ID (NCBI): 794
• RRID: AB_2919687
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.