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  • KD/KO Validated

Caspase 8/p43/p18 Monoclonal antibody

Caspase 8/p43/p18 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human and More (6)







Cat no : 66093-1-Ig


Apoptotic cysteine protease, Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, Caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, HEK-293 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66093-1-Ig is Caspase 8/p43/p18 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, chicken, sheep, monkey, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Caspase 8/p43/p18 fusion protein Ag20524
Full Name caspase 8, apoptosis-related cysteine peptidase
Calculated Molecular Weight 538 aa, 62 kDa
Observed Molecular Weight 53-57 kDa, 32-45 kDa, 18 kDa
GenBank Accession NumberBC028223
Gene Symbol CASP8
Gene ID (NCBI) 841
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.


Product Specific Protocols
WB protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
IHC protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
IF protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
IP protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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