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  • KD/KO Validated

Caspase 8/p43/p18 Monoclonal antibody

Caspase 8/p43/p18 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (5)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B9H8

Cat no : 66093-1-Ig

Synonyms

Apoptotic cysteine protease, Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, Caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, HEK-293 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66093-1-Ig is Caspase 8/p43/p18 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, chicken, monkey, sheep
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Caspase 8/p43/p18 fusion protein Ag20524
Full Name caspase 8, apoptosis-related cysteine peptidase
Calculated molecular weight 538 aa, 62 kDa
Observed molecular weight 53-57 kDa, 32-45 kDa, 18 kDa
GenBank accession numberBC028223
Gene symbol CASP8
Gene ID (NCBI) 841
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.

Protocols

Product Specific Protocols
WB protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
IHC protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
IF protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
IP protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.

Authors - Dianrong Li
  • KO Validated
mouseWB

J Pineal Res

Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota.

Authors - Wenkai Ren
humanWB,IF

Chem Sci

Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay.

Authors - Meng Liu
chickenWB

J Hazard Mater

The role of ATF6 in Cr(VI)-induced apoptosis in DF-1 cells.

Authors - Shuo Zhang
mouseIF

Elife

RIPK1-RIPK3-MLKL-dependent necrosis promotes the aging of mouse male reproductive system.

Authors - Dianrong Li
humanWB,IP

EMBO Rep

Bclaf1 regulates c-FLIP expression and protects cells from TNF-induced apoptosis and tissue injury.

Authors - Rui Zhang