Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66093-1-Ig targets Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, sheep |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20524 Product name: Recombinant human Caspase 8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-270 aa of BC028223 Sequence: MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGTV Predict reactive species |
| Full Name | caspase 8, apoptosis-related cysteine peptidase |
| Calculated Molecular Weight | 538 aa, 62 kDa |
| Observed Molecular Weight | 53-57 kDa, 32-45 kDa, 18 kDa |
| GenBank Accession Number | BC028223 |
| Gene Symbol | Caspase 8 |
| Gene ID (NCBI) | 841 |
| RRID | AB_11232214 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14790 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Caspase 8/P43/P18 antibody 66093-1-Ig | Download protocol |
| IHC protocol for Caspase 8/P43/P18 antibody 66093-1-Ig | Download protocol |
| IP protocol for Caspase 8/P43/P18 antibody 66093-1-Ig | Download protocol |
| WB protocol for Caspase 8/P43/P18 antibody 66093-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Caspase 8/P43/P18 Monoclonal antibody (2B9H8) (Cat# 66093-1-Ig) has 115 total citations. Citations span over 80 journals, including high-impact titles such as Nature Communications, Cell Stem Cell, Science Immunology, Molecular Cell, Cell Research, EMBO Reports, and eLife. Associated research fields include apoptosis, necroptosis, pyroptosis, PANoptosis, cancer biology, inflammation, liver disease, neurodegeneration, ferroptosis, and immunology.
| Species | Application | Title |
|---|---|---|
Cell Res Targeting PTPN13 with 11-amino-acid peptides of C-terminal APC prevents immune evasion of colorectal cancer. | ||
Cancer Commun (Lond) Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies | ||
Cell Stem Cell Nynrin preserves hematopoietic stem cell function by inhibiting the mitochondrial permeability transition pore opening | ||
Nat Commun E4F1 coordinates pyruvate metabolism and the activity of the elongator complex to ensure translation fidelity during brain development | ||
J Adv Res Tetrahydrocurcumin targets TRIP13 inhibiting the interaction of TRIP13/USP7/c-FLIP to mediate c-FLIP ubiquitination in triple-negative breast cancer | ||
