Tested Applications
| Positive WB detected in | pig colon tissue, human cervical cancer tissue, human placenta tissue, MG-63 cells, pig lung tissue, pig skin tissue |
| Positive IHC detected in | human breast cancer tissue, human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 183 publications below |
| IHC | See 57 publications below |
| IF | See 59 publications below |
| CoIP | See 1 publications below |
Product Information
67288-1-Ig targets Collagen Type I in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, pig, rabbit, zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | collagen, type I, alpha 1 |
| Calculated Molecular Weight | 139 kDa |
| Observed Molecular Weight | 100-140 kDa，200kDa |
| GenBank Accession Number | NM_000088 |
| Gene Symbol | COL1A1 |
| Gene ID (NCBI) | 1277 |
| RRID | AB_2882554 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P02452 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727). It is reported that the molecular weight is 200kDa(PMID: 28526934).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Collagen Type I antibody 67288-1-Ig | Download protocol |
| IHC protocol for Collagen Type I antibody 67288-1-Ig | Download protocol |
| IF protocol for Collagen Type I antibody 67288-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Biodegradable Piezoelectric-Conductive Integrated Hydrogel Scaffold for Repair of Osteochondral Defects | ||
J Hematol Oncol Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma | ||
Nat Cell Biol Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis. | ||
Adv Sci (Weinh) Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner | ||
Small M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation | ||
Eur Respir J Intracellular hydroxyproline imprinting following resolution of bleomycin-induced pulmonary fibrosis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Niky (Verified Customer) (02-26-2024) | Arrived very quickly on ice. However, didnt work for my tissue type
|
FH Udesh (Verified Customer) (12-22-2023) | Worked well for IF at 1:400
|
FH Niklas (Verified Customer) (11-08-2022) | great staining with even a high dilution factor
|
FH Ren Jie (Verified Customer) (06-22-2022) | Does not work for pepsin digested human collagen I
|
FH Hala (Verified Customer) (02-26-2021) | very good
|
FH Hala (Verified Customer) (02-23-2021) | very good
|
