Tested Applications
| Positive IF-P detected in | human colon cancer tissue, human breast cancer tissue, mouse skin tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-67288 targets Collagen Type I in IF-P applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | collagen, type I, alpha 1 |
| Calculated Molecular Weight | 139 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | NM_000088 |
| Gene Symbol | COL1A1 |
| Gene ID (NCBI) | 1277 |
| RRID | AB_2920107 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P02452 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Collagen Type I antibody CL594-67288 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Collagen Type I Monoclonal antibody (1E9A7), catalog number CL594-67288, has 4 citations. They appear in Int J Biol Sci, J Invest Dermatol, Diabetol Metab Syndr, and FASEB J. The research fields span hepatocellular carcinoma lipid metabolism, skin aging and collagen fragmentation, diabetic kidney fibrosis, and hepatic stellate cell–mediated thrombin formation and fibrosis. All four citations used the antibody for immunofluorescence (IF) applications across human, pig, and mouse species.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma | ||
J Invest Dermatol Oxidative Fragmentation of Collagen in Skin: Relevance to Skin Aging and Skin Diseases. | ||
Diabetol Metab Syndr SRPK1 is a significant factor in driving the progression of diabetic kidney fibrosis. | ||
FASEB J Prorenin in Hepatic Stellate Cell Extracellular Vesicles Induces Platelet-Dependent Thrombin Formation and Release of Profibrotic TGF-β. |
Reviews
What customers say
The single review for CL594-67288 is a 5-star rating from an Immunofluorescence application on Y201-MSC cells at a 1:500 dilution. The user reported strong signal intensity, noting they would use a higher dilution next time to reduce it. Overall, the antibody performed well, delivering robust staining with only a minor suggestion to adjust dilution for optimal results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Areli (Verified Customer) (03-14-2023) | I would use a higher dilution next time as the signal was high.
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