CoraLite®594-conjugated Collagen Type I Monoclonal antibody

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  IF Staining of human colon cancer using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200.

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  IF Staining of mouse skin using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200.

Collagen Type I Monoclonal Antibody for IF-P

Cat No. CL594-67288

Clone No.1E9A7

Publications(1)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF-P

Synonyms

COL1A1, col1, COL I, Alpha-1 type I collagen, Alpha 1 type I collagen

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Tested Applications

• Positive IF-P detected in: human colon cancer tissue, human breast cancer tissue, mouse skin tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-67288 targets Collagen Type I in IF-P applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: collagen, type I, alpha 1
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: NM_000088
• Gene Symbol: COL1A1
• Gene ID (NCBI): 1277
• RRID: AB_2920107
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P02452
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Int J Biol Sci; Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma; Authors - Jingwei Cai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Areli (Verified Customer) (03-14-2023)

I would use a higher dilution next time as the signal was high.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Y201-MSC cells

CL594 Collagen Type I Antibody Immunofluorescence validation (1:500 dilution) in Y201-MSC cells (Cat no:CL594-67288)