Collagen Type I Monoclonal antibody, PBS Only

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  WB analysis of pig colon using 67288-1-Ig (same clone as 67288-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67288-1-Ig (same clone as 67288-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67288-1-Ig (same clone as 67288-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

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  WB analysis of human cervical cancer using 67288-1-Ig (same clone as 67288-1-PBS)

  human cervical cancer tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig lung using 67288-1-Ig (same clone as 67288-1-PBS)

  pig lung tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig skin using 67288-1-Ig (same clone as 67288-1-PBS)

  pig skin tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67288-1-Ig (same clone as 67288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67288-1-Ig (same clone as 67288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67288-1-Ig (same clone as 67288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67288-1-Ig (same clone as 67288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody (67288-1-Ig, Clone: 1E9A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody (67288-1-Ig, Clone: 1E9A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

Collagen Type I Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67288-1-PBS

Clone No.1E9A7

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

COL1A1, 1E9A7, Alpha 1 type I collagen, Alpha-1 type I collagen, COL I

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Product Information

67288-1-PBS targets Collagen Type I in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: collagen, type I, alpha 1
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 100-140 kDa，200kDa
• GenBank Accession Number: NM_000088
• Gene Symbol: COL1A1
• Gene ID (NCBI): 1277
• RRID: AB_2882554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P02452
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727). It is reported that the molecular weight is 200kDa(PMID: 28526934).