Cystatin SN/CST1 Recombinant antibody

• 

  WB analysis using 85403-1-RR

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 85403-1-RR (CST1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Cystatin SN/CST1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85403-1-RR

Clone No.242888B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CST1, Cystain SA I, Cystain-SA-I, Cystatin 1, cystatin SN

---

Tested Applications

• Positive WB detected in: MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85403-1-RR targets Cystatin SN/CST1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cystatin SN/CST1 fusion protein Ag8862
• Full Name: cystatin SN
• Calculated Molecular Weight: 141 aa, 16 kDa
• Observed Molecular Weight: 14-16 kDa
• GenBank Accession Number: BC021225
• Gene Symbol: CST1
• Gene ID (NCBI): 1469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01037
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CST1, or cystatin SN, is a gene in humans that encodes a secreted protein belonging to the type 2 cystatin superfamily, which includes CST1, CST2, CST3, CST4, and CST5. These proteins are cysteine proteinase inhibitors found in various human fluids and secretions, where they appear to provide protective functions . CST1 is specifically found in saliva, tears, urine, and seminal fluid, and it plays a role in inhibiting the activity of cysteine proteases. CST1 has been implicated in various pathological processes, including tumor invasion and metastasis. Overexpression of CST1 has been observed in several types of cancer, such as lung, breast, colorectal, and gastric cancer, suggesting its role in the proliferation, invasion, and metastasis of these tumors.