Cytokeratin 15 Monoclonal antibody

Cytokeratin 15 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

6B4F8

Cat no : 60247-1-Ig

Synonyms

CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB



Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman oesophagus cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60247-1-Ig is Cytokeratin 15 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 15 fusion protein Ag0185
Full Name keratin 15
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC002641
Gene symbol KRT15
Gene ID (NCBI) 3866
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
IHC protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
IF protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
FC protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols