Cytokeratin 20 Polyclonal antibody
Cytokeratin 20 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat, rabbit
WB, IHC, IF, FC, ELISA
Cat no : 17329-1-AP
Validation Data Gallery
|Positive WB detected in||COLO 320 cells, HeLa cells, MCF-7 cells, HT-29 cells|
|Positive IHC detected in||human colon tissue, human appendicitis tissue, human stomach cancer tissue, human urothelial carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HT-29 cells, Rabbit colon tissue, HeLa cells|
|Positive FC detected in||HT-29 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|WB||See 2 publications below|
|IHC||See 1 publications below|
|IF||See 5 publications below|
17329-1-AP targets Cytokeratin 20 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
|Tested Reactivity||human, mouse, rat, rabbit|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytokeratin 20 fusion protein Ag11308|
|Full Name||keratin 20|
|Calculated molecular weight||424 aa, 48 kDa|
|Observed molecular weight||46 kDa|
|GenBank accession number||BC031559|
|Gene ID (NCBI)||54474|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
|Product Specific Protocols|
|WB protocol for Cytokeratin 20 antibody 17329-1-AP||Download protocol|
|IHC protocol for Cytokeratin 20 antibody 17329-1-AP||Download protocol|
|IF protocol for Cytokeratin 20 antibody 17329-1-AP||Download protocol|
|FC protocol for Cytokeratin 20 antibody 17329-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons.
Nat Cell Biol
LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.
5-hydroxytryptamine produced by enteric serotonergic neurons initiates colorectal cancer stem cell self-renewal and tumorigenesis.
A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium.
LGR4 cooperates with PrPc to endow the stemness of colorectal cancer stem cells contributing to tumorigenesis and liver metastasis.
DC - SIGNR by influencing the lncRNA HNRNPKP2 upregulates the expression of CXCR4 in gastric cancer liver metastasis.