DCX Recombinant monoclonal antibody

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• DCX Recombinant monoclonal antibody (84595-4-RR)

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  WB analysis of fetal human brain using 84595-4-RR

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 84595-4-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 84595-4-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84595-4-RR (DCX antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84595-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84595-4-RR against Human DCX were performed. The affinity constant is 1.23 nM.

This DCX antibody is a rabbit recombinant antibody that targets DCX in WB, IHC, ELISA and is suitable for human, rat samples.

Cat No. 84595-4-RR

Clone No.241985H1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, fetal human brain tissue
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84595-4-RR targets DCX in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4945

  Product name: Recombinant human DCX protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-360 aa of BC027925

  Sequence: MELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM

• Full Name: doublecortin
• Calculated Molecular Weight: 402 aa, 45 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC027925
• Gene Symbol: DCX
• Gene ID (NCBI): 1641
• RRID: AB_3743594
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O43602
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.