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Desmin Polyclonal antibody

Desmin Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 16520-1-AP


Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin

Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human appendix tissue, human skeletal muscle tissue, human placenta tissue, human colon tissue, human hysteromyoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells, mouse heart tissue, rat heart tissue
Positive FC detected inC2C12 cell

Recommended dilution

Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16520-1-AP targets Desmin in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Desmin fusion protein Ag9742
Full Name desmin
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight 52-53 kDa
GenBank Accession NumberBC032116
Gene Symbol DES
Gene ID (NCBI) 1674
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.


Product Specific Protocols
WB protocol for Desmin antibody 16520-1-APDownload protocol
IHC protocol for Desmin antibody 16520-1-APDownload protocol
IF protocol for Desmin antibody 16520-1-APDownload protocol
IP protocol for Desmin antibody 16520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kamal (Verified Customer) (01-26-2024)

Antibody diluted in 1X PBS, dilution 1:50000, worked well for western blot analysis in mouse liver tissues.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:50000
  • Cell Tissue Type: Mouse liver tissue

Brice-Emmanuel (Verified Customer) (09-12-2023)

Perfect if you want to see bandes of degraded desmin. I diluted the antibody 1:5000 in NFDM5%.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000
  • Cell Tissue Type: Mouse Heart Tissue
Desmin Antibody Western Blot validation (1/5000 dilution) in Mouse Heart Tissue (Cat no:16520-1-AP)

Víctor (Verified Customer) (06-12-2023)

It is a good marker for muscle cells. Indeed, sarcomeric structures can be seen with this antibody. High specificity and low background.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Skeletal muscle cells obtained by direct cell reprogramming approaches
Desmin Antibody Immunofluorescence validation (1:500 dilution) in Skeletal muscle cells obtained by direct cell reprogramming approaches (Cat no:16520-1-AP)

Sarah (Verified Customer) (11-16-2019)

This antibody worked well for both WB (1:4000 for 1 hour) and IP (1.6ug Ab/ 500ug Protein)

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: WB- 1:4000, IP- 1.6ug
  • Cell Tissue Type: C2C12 Cells
Desmin Antibody Western Blot,Immunoprecipitation, validation (WB- 1:4000, IP- 1.6ug dilution) in C2C12 Cells (Cat no:16520-1-AP)