Validation Data Gallery
|Positive WB detected in||C2C12 cells, human heart tissue, mouse heart tissue, rat heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human heart tissue, human appendix tissue, human skeletal muscle tissue, human placenta tissue, human colon tissue, human hysteromyoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C2C12 cells, mouse heart tissue, rat heart tissue|
|Positive FC detected in||C2C12 cell|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
16520-1-AP targets Desmin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Desmin fusion protein Ag9742|
|Calculated molecular weight||470 aa, 54 kDa|
|Observed molecular weight||52-53 kDa|
|GenBank accession number||BC032116|
|Gene ID (NCBI)||1674|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.
|Product Specific Protocols|
|WB protocol for Desmin antibody 16520-1-AP||Download protocol|
|IHC protocol for Desmin antibody 16520-1-AP||Download protocol|
|IF protocol for Desmin antibody 16520-1-AP||Download protocol|
|IP protocol for Desmin antibody 16520-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Brice-Emmanuel (Verified Customer) (09-12-2023)
Perfect if you want to see bandes of degraded desmin. I diluted the antibody 1:5000 in NFDM5%.
Víctor (Verified Customer) (06-12-2023)
It is a good marker for muscle cells. Indeed, sarcomeric structures can be seen with this antibody. High specificity and low background.
Sarah (Verified Customer) (11-16-2019)
This antibody worked well for both WB (1:4000 for 1 hour) and IP (1.6ug Ab/ 500ug Protein)