Multi-rAb® DIAPH3 Multi-Recombinant antibody

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  WB analysis using RMX00041

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00041 (DIAPH3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using RMX00041

  IP result of anti-DIAPH3 (IP:RMX00041, 4ug; Detection:RMX00041 1:8000) with HeLa cells lysate 1080 ug.

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  IF Staining of HeLa using RMX00041

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DIAPH3 antibody (RMX00041) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DIAPH3 Multi-Recombinant Antibody for WB, IF/ICC, IP, ELISA

Cat No. RMX00041

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

DIAP3, Diaphanous-related formin-3, DRF3, MDia2

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00041 targets DIAPH3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: diaphanous homolog 3 (Drosophila)
• Calculated Molecular Weight: 137 kDa
• Observed Molecular Weight: 137 kDa
• GenBank Accession Number: BC048963
• Gene Symbol: DIAPH3
• Gene ID (NCBI): 81624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q9NSV4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.