DMT1 Recombinant antibody

DMT1 Recombinant Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1I13

Cat no : 81609-1-RR

Synonyms

DCT1, Divalent cation transporter 1, Divalent metal transporter 1, DMT 1, DMT1, FLJ37416, NRAMP 2, NRAMP2, SLC11A2



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, HuH-7 cells, Caco-2 cells, Neuro-2a cells, HSC-T6 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

81609-1-RR targets DMT1 in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DMT1 fusion protein Ag14314
Full Name solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
Calculated molecular weight 568 aa, 62 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC002592
Gene symbol SLC11A2
Gene ID (NCBI) 4891
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that plays a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including brain, kidney, testis, duodenum and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.

Protocols

Product Specific Protocols
WB protocol for DMT1 antibody 81609-1-RRDownload protocol
IP protocol for DMT1 antibody 81609-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Amandine (Verified Customer) (02-06-2024)

With 20ug of protein denatured or not, 1H30 RT 1/10000, secondary antibody 2h 1/5000. Chemiluminescence revelation, 6min of exposition

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/10000
  • Cell Tissue Type: ImCC
DMT1 Antibody Western Blot validation (1/10000 dilution) in ImCC (Cat no:81609-1-RR)