DNA-RNA Hybrid Recombinant monoclonal antibody

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  IHC staining of human ovary tumor using 85855-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 85855-1-RR (DNA-RNA Hybrid antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 85855-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85855-1-RR (DNA-RNA Hybrid antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 85855-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85855-1-RR (DNA-RNA Hybrid antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 85855-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNA-RNA Hybrid antibody (85855-1-RR, Clone: 250863A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FBL antibody (66985-1-Ig, Clone: 3A9E8, red).

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  IF Staining of HepG2 using 85855-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNA-RNA Hybrid antibody (85855-1-RR, Clone: 250863A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FBL antibody (66985-1-Ig, Clone: 3A9E8, red).

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  FC experiment of HEK-293T using 85855-1-RR

  1x10^6 HEK-293T cells were intracellularly stained with 0.2 ug N/a DNA-RNA Hybrid Recombinant Antibody (85855-1-RR, Clone:250863A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Dot Blot experiment of / using 85855-1-RR

  Dot blot analysis was used to confirm the specificity of 85855-1-RR DNA-RNA Hybrid antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (ng) spotted is indicated next to each row.

DNA-RNA Hybrid Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Dot blot, ELISA

Cat No. 85855-1-RR

Clone No.250863A1

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

IHC, IF/ICC, FC (Intra), Dot blot, ELISA

Synonyms

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Tested Applications

• Positive IHC detected in: human placenta tissue, human ovary tumor tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells
• Positive FC (Intra) detected in: HEK-293T cells
• Positive Dot blot detected in: / /

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85855-1-RR targets DNA-RNA Hybrid in IHC, IF/ICC, FC (Intra), Dot blot, ELISA applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: DNA-RNA Hybrid
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA-RNA Hybrid antibodies are primarily used to study R-loops. Theoretically, they do not bind to single-stranded DNA, double-stranded DNA, or RNA. R-loops are DNA-RNA hybrid structures formed during transcription and are closely related to genome stability, DNA replication, DNA repair, and cancer development.