DNAJC19 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNAJC19 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 12096-1-AP

Synonyms

TIMM14, TIM14

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Validation Data Gallery

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  WB analysis of HeLa using 12096-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12096-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12096-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12096-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12096-1-AP

  IP result of anti-DNAJC19 (IP:12096-1-AP, 4ug; Detection:12096-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IF Staining of HepG2 using 12096-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAJC19 antibody (12096-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, human lung tissue
• Positive IP detected in: mouse heart tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• FC: See 1 publications below

Product Information

12096-1-AP targets DNAJC19 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJC19 fusion protein Ag2739
• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 19
• Calculated Molecular Weight: 116 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC009702
• Gene Symbol: DNAJC19
• Gene ID (NCBI): 131118
• RRID: AB_2094914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC19 is a mitochondrial cochaperone that interacts with HSP70 chaperones through its conserved J-domain. As a transmembrane protein, DNAJC19 is strongly associated with the inner mitochondrial membrane. Mutations in DNAJC19 cause dilated cardiomyopathy with ataxia (DCMA). Recently DNAJC19 has been reported as interactor of PHB complex to regulate cardiolipin remodeling, which addressed the link of DNAJC19 to cardiomyopathy. This antibody is specific to DNAJC19 and had been tested by siRNA.(24856930)

Publications

Species	Application	Title
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	Cell Metab; DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.; Authors - Ricarda Richter-Dennerlein ; KD Validated
human	WB	J Cell Biol; OMA1 protease eliminates arrested protein import intermediates upon mitochondrial depolarization; Authors - Magda Krakowczyk
human	WB	Hum Mol Genet; Defining functional classes of Barth syndrome mutation in humans.; Authors - Ya-Wen Lu
human		Stem Cell Res; Generation of two patient-derived iPSC lines from siblings (LIBUCi001-A and LIBUCi002-A) and a genetically modified iPSC line (JMUi001-A-1) to mimic dilated cardiomyopathy with ataxia (DCMA) caused by a homozygous DNAJC19 mutation.; Authors - Anna Janz
human	WB	Cell Rep; The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.; Authors - Michelle Grace Acoba