ChromoTek DYKDDDDK Immunoprecipitation Starter Pack

The DYKDDDDK Immunoprecipitation Starter Pack is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

Specificity

1x and 3xFlag®-tag

Applications

IP

Conjugate

Agarose

Cat no : ffap

Synonyms

DYKDDDDK, Flag



Product Information

The DYKDDDDK Immunoprecipitation Starter Pack is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

ApplicationsIP
HostMouse
ComponentsDYKDDDDK Fab-Trap™ Agarose (20 reactions, ffa-20)
3xDYKDDDDK-peptide (1mg, fp-1)
DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL, 20543-1-AP-20).
SpecificitySpecifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). Compatible with N-, C- terminal or internal tagging
Binding capacity12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry
Bead size90 µm (cross-linked 4 % agarose beads)
Elution buffer3xDYKDDDDK-peptide (fp-1)
SDS sample buffer
0.2 M glycine pH 2.5
Storage buffer1x PBS, Preservative: 0.09 % sodium azide
Storage conditionsUpon receipt store at +4°C. Do not freeze!
StabilityStable for 1 year upon receipt.
ShipmentShipped at ambient temperature.