ChromoTek DYKDDDDK Immunoprecipitation Starter Pack

Validation Data Gallery

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  DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

The DYKDDDDK Immunoprecipitation Starter Pack is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

Cat No. ffap

Specificity

1x and 3x Flag®-tag

Applications

IP

Type

Fab-fragment

Conjugate

Agarose

Synonyms

DYKDDDDK, Flag

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Product Information

The DYKDDDDK Immunoprecipitation Starter Pack is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

• Description: The DYKDDDDK Immunoprecipitation Starter Pack is used to immunoprecipitate, elute, and detect 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
• Applications: IP
• Host: Mouse
• Components: DYKDDDDK Fab-Trap™ Agarose (20 reactions, ffa-20); 3xDYKDDDDK-peptide (1mg, fp-1); DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL, 20543-1-AP-20).
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Specificity/Target: Specifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). Compatible with N-, C- terminal or internal tagging
• Binding capacity: 12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry
• Elution buffer: 3xDYKDDDDK-peptide (fp-1) ; SDS sample buffer; 0.2 M glycine pH 2.5
• Storage buffer: 1x PBS, Preservative: 0.09 % sodium azide
• Storage Condition: Upon receipt store at +4°C. Do not freeze!
• Stability: Stable for 1 year upon receipt.
• Shipment: Shipped at ambient temperature.

Kit components

• Component: Description
• ffa-20: DYKDDDDK Fab-Trap™ Agarose (20 reactions)
• fp-1: 3xDYKDDDDK-peptide (1mg)
• 20543-1-AP: DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL)

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