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ECHS1 Monoclonal antibody

ECHS1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b









Cat no : 66117-1-Ig


ECHS1, Enoyl CoA hydratase 1, SCEH

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human testis tissue, PC-3 cells, HEK-293 cells, L02 cells, COLO 320 cells, T47D cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66117-1-Ig targets ECHS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ECHS1 fusion protein Ag16775
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC008906
Gene Symbol ECHS1
Gene ID (NCBI) 1892
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)


Product Specific Protocols
WB protocol for ECHS1 antibody 66117-1-IgDownload protocol
IHC protocol for ECHS1 antibody 66117-1-IgDownload protocol
IF protocol for ECHS1 antibody 66117-1-IgDownload protocol
IP protocol for ECHS1 antibody 66117-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Proteomics

Up-regulation of fatty acid oxidation in the ligament as a contributing factor of ankylosing spondylitis: A comparative proteomic study.

Authors - Wei-Dong Xu

Ann Clin Transl Neurol

Deficiency of ECHS1 causes mitochondrial encephalopathy with cardiac involvement.

Authors - Tobias B Haack

FEBS Open Bio

The role of SAMM50 in non-alcoholic fatty liver disease: from genetics to mechanisms.

Authors - Zuyin Li

Cell Death Dis

ECHS1, an interacting protein of LASP1, induces sphingolipid-metabolism imbalance to promote colorectal cancer progression by regulating ceramide glycosylation.

Authors - Rui Li
  • KD Validated