|Positive WB detected in||HeLa cells, human testis tissue, PC-3 cells, HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human prostate cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:1000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66117-1-Ig is ECHS1 Fusion Protein expressed in E. coli.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||ECHS1 fusion protein Ag16775|
|Full Name||enoyl Coenzyme A hydratase, short chain, 1, mitochondrial|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC008906|
|Gene ID (NCBI)||1892|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)
FEBS Open Bio
The role of SAMM50 in non-alcoholic fatty liver disease: from genetics to mechanisms.
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Deficiency of ECHS1 causes mitochondrial encephalopathy with cardiac involvement.
Up-regulation of fatty acid oxidation in the ligament as a contributing factor of ankylosing spondylitis: A comparative proteomic study.