EGFR Recombinant monoclonal antibody

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  WB analysis using 83806-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83806-1-RR (EGFR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 83806-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR-Specific antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A431 using 83806-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR-Specific antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HaCaT using 83806-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using EGFR antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A431 using 83806-1-RR

  1x10^6 A431 cells (left) or Jurkat cells (right) were intracellularly stained with 0.4 ug EGFR Recombinant antibody (83806-1-RR, Clone: 240603F10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA.

EGFR Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83806-1-RR

Clone No.240603F10

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

EGFR-Specific, 240603F10, EGF R, EGF Receptor , Epidermal growth factor receptor

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IF/ICC detected in: A431 cells, HaCaT cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

83806-1-RR targets EGFR in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 145-165 kDa
• GenBank Accession Number: NM_005228
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• RRID: AB_3671393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P00533
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IHC	Int Immunopharmacol; The matrix of exosomes: Decoding the role of miRNA in macrophage polarization and oxidative stress; Authors - Yuanyuan Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (09-24-2025)

EGFR (in green) worked perfectly marking a specific subtype of astrocytes in human brain cortical dysplasia tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

EGFR Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:83806-1-RR)