EIF4E Polyclonal antibody, PBS Only

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  WB analysis using 29712-1-AP (same clone as 29712-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29712-1-AP (EIF4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29712-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29712-1-AP (same clone as 29712-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29712-1-AP (EIF4E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29712-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 29712-1-AP (same clone as 29712-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29712-1-AP (EIF4E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29712-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 29712-1-AP (same clone as 29712-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF4E antibody (29712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29712-1-PBS in a different storage buffer formulation.

EIF4E Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 29712-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

29712-1-PBS targets EIF4E in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: eukaryotic translation initiation factor 4E
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 26-29 kDa
• GenBank Accession Number: BC012611
• Gene Symbol: EIF4E
• Gene ID (NCBI): 1977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P06730
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins.