Tested Applications
| Positive WB detected in | HEK-293 cells, HSC-T6 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29712-1-AP targets EIF4E in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Rat samples.
| Tested Reactivity | Human, Rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | eukaryotic translation initiation factor 4E |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 26-29 kDa |
| GenBank Accession Number | BC012611 |
| Gene Symbol | EIF4E |
| Gene ID (NCBI) | 1977 |
| RRID | AB_3086152 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06730 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF4E antibody 29712-1-AP | Download protocol |
| IHC protocol for EIF4E antibody 29712-1-AP | Download protocol |
| WB protocol for EIF4E antibody 29712-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF4E Polyclonal antibody (Cat# 29712-1-AP) has 2 total citations. These appear in Cell Death & Disease (2024) and International Journal of Biological Macromolecules (2025). The research fields covered include ferroptosis-mediated breast cancer suppression via p53 acetylation, and kinase-independent Bcr-Abl function in chronic myeloid leukemia. Both studies utilized the antibody for Western blotting in human samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis FXR deficiency induced ferroptosis via modulation of the CBP-dependent p53 acetylation to suppress breast cancer growth and metastasis
| ||
Int J Biol Macromol A kinase-independent Bcr-Abl function mediating an Hsp70-Bim protein-protein interaction in chronic myeloid leukemia |
