ELA2 Polyclonal antibody

ELA2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27642-1-AP

Synonyms

Bone marrow serine protease, ELA2, ELANE, Elastase 2, elastase 2, neutrophil, GE, HLE, HNE, Human leukocyte elastase, Medullasin, NE, Neutrophil elastase, PMN E, PMN elastase



Tested Applications

Positive WB detected inTHP-1 cells, U-937 cells
Positive IHC detected inhuman lymphoma tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27642-1-AP targets ELA2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivitymouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELA2 fusion protein Ag26634
Full Name elastase 2, neutrophil
Calculated Molecular Weight 267 aa, 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC074816
Gene Symbol ELA2
Gene ID (NCBI) 1991
RRIDAB_2918126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation(PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation)(PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration(PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for ELA2 antibody 27642-1-APDownload protocol
IHC protocol for ELA2 antibody 27642-1-APDownload protocol
IF protocol for ELA2 antibody 27642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Cell Mol Biol Lett

Obacunone alleviates ferroptosis during lipopolysaccharide-induced acute lung injury by upregulating Nrf2-dependent antioxidant responses.

Authors - Jin Li
bovineIF

Animals (Basel)

Effects of Hormone, NEFA and SCFA on the Migration of Neutrophils and the Formation of Neutrophil Extracellular Traps in Dairy Cows.

Authors - Guanxin Lv
mouseWB

Cancer Immunol Res

Tumor cell-derived microparticles induced by methotrexate augment T-cell antitumor responses by down-regulating expression of PD-1 in neutrophils

Authors - Pingwei Xu
ratIHC

Front Pharmacol

RNA-sequencing approach for exploring the protective mechanisms of dexmedetomidine on pancreatic injury in severe acute pancreatitis

Authors - Jiaqi Yao