ELF2 Polyclonal antibody

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• ELF2 Polyclonal antibody (12499-1-AP)

• 

  WB analysis of A2780 using 12499-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12499-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12499-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 12499-1-AP

  IP result of anti-ELF2 (IP:12499-1-AP, 4ug; Detection:12499-1-AP 1:500) with A2780 cells lysate 960ug.

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  IF Staining of MCF-7 using 12499-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ELF2 antibody (12499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

ELF2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 12499-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ChIP, ELISA

Synonyms

E74 like factor 2, E74-like factor 2, ELF2A, ETS-related transcription factor Elf-2, EU32

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Tested Applications

• Positive WB detected in: A2780 cells, human brain tissue, human liver tissue
• Positive IP detected in: A2780 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

12499-1-AP targets ELF2 in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3170

  Product name: Recombinant human ELF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-353 aa of BC034951

  Sequence: MATSLHEGPTNQLDLLIRAVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPVEVFVPPCVSTPEFIHAAMRPDVITETVVEVSTEESEPMDTSPIPTSPDSHEPMKKKKVGRKPKTQQSPISNGSPELGIKKKPREGKGNTTYLWEFLLDLLQDKNTCPRYIKWTQREKGIFKLVDSKAVSKLWGKHKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKDMPKNIVVIDDDKSETCNEDLAGTTDEKSLERVSLSAESLLKAASSVRSGKNSSPINCSRAEKGVARVVNITSPGHDASSRSPTTTASVSATAAPRTVRVAMQVPVVMTSLGQKISTVAVQSV

• Full Name: E74-like factor 2 (ets domain transcription factor)
• Calculated Molecular Weight: 533 aa, 57 kDa
• Observed Molecular Weight: 63-64 kDa, 56-57 kDa
• GenBank Accession Number: BC034951
• Gene Symbol: ELF2
• Gene ID (NCBI): 1998
• RRID: AB_10642956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15723
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ets-1 is the prototype member of a family of genes firstly identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family have varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. ELF2 belongs to the Ets-1 family. ELF2 exists several isoforms and the range of molucular weight of isoform is about 54-66 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
goat	WB	Front Cell Dev Biol; A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development.; Authors - Yue Zhang ; KD Validated
human	WB	Cell Biol Int; Inhibition of the miR-193b-3p protects against oxidized low-density lipoprotein-induced HUVECs injury by upregulating ALDH2.; Authors - Yunpeng Bai
mouse	WB	Ren Fail; ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyte.; Authors - Shizhu Yuan
human	ChIP	Nat Commun; Allelic effects on KLHL17 expression underlie a pancreatic cancer genome-wide association signal at chr1p36.33; Authors - Katelyn E Connelly
human	WB	Cell Death Dis; Loss of ELF2 drives topotecan resistance in retinoblastoma revealed by genome-wide CRISPR-Cas9 screening.; Authors - Jingyi Jiang ; KO Validated
human	IF	Mol Cell; Targeted citrullination enables p53 binding to non-canonical sites.; Authors - Alexandra Indeglia