Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, COLO 320 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10351-1-AP targets Emerin in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0236 Product name: Recombinant human EMD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 66-252 aa of BC000738 Sequence: STRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGN Predict reactive species |
| Full Name | emerin |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC000738 |
| Gene Symbol | EMD |
| Gene ID (NCBI) | 2010 |
| RRID | AB_2100056 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50402 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Emerin antibody 10351-1-AP | Download protocol |
| IF protocol for Emerin antibody 10351-1-AP | Download protocol |
| IHC protocol for Emerin antibody 10351-1-AP | Download protocol |
| WB protocol for Emerin antibody 10351-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Emerin Polyclonal antibody (Cat# 10351-1-AP) has 41 total citations appearing in journals including Nature, Science, Cell, Cancer Cell, Nature Cell Biology, Nature Communications, Molecular Cell, Developmental Cell, Journal of Cell Biology, Scientific Reports, PLoS Genetics, Carcinogenesis, and others. Associated research fields encompass nuclear envelope biology, laminopathies (Emery-Dreifuss muscular dystrophy), cancer biology (breast, prostate, colorectal), chromatin organization, cell cycle regulation, and nuclear structural dynamics.
| Species | Application | Title |
|---|---|---|
Cancer Cell Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite | ||
Science A p62-dependent rheostat dictates micronuclei catastrophe and chromosome rearrangements | ||
Nat Cell Biol LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion |
Reviews
What customers say
The single review for this antibody comes from a researcher who used it for Western Blot at a 1:1000 dilution on MDCK GII cells. They rated it 5 stars and reported that the antibody recognized a clear band at the correct expected molecular weight of Emerin, indicating reliable specificity and performance for their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Joleen (Verified Customer) (06-12-2019) | Antibody recognizes clear band at the corrected expected molecular weight of Emerin.
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