ENO1 Monoclonal antibody

ENO1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat, Pig

Applications

WB

Conjugate

Unconjugated

CloneNo.

1D6E8

Cat no : 67187-1-Ig

Synonyms

Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH

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Validation Data Gallery

• 

  WB analysis using 67187-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67187-1-Ig (ENO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, PC-3 cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

Sample-dependent, check data in validation data gallery

Product Information

67187-1-Ig targets ENO1 in WB applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: ENO1 fusion protein Ag17518
• Full Name: enolase 1, (alpha)
• Calculated molecular weight: 47 kDa
• Observed molecular weight: 47 kDa
• GenBank accession number: BC015641
• Gene symbol: ENO1
• Gene ID (NCBI): 2023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. It is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. ENO1 may also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. It stimulates immunoglobulin production. (PMID:2005901).