ENO1 Monoclonal antibody

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  WB analysis of HEK-293 using 67187-1-Ig

  WB result of ENO1 antibody (67187-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HEK-293 cells.

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  WB analysis using 67187-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67187-1-Ig (ENO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67187-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENO1 antibody (67187-1-Ig, Clone: 1D6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• Featured Product
• KD/KO Validated

ENO1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67187-1-Ig

Clone No.1D6E8

Publications(1)

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, PC-3 cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67187-1-Ig targets ENO1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: ENO1 fusion protein Ag17518
• Full Name: enolase 1, (alpha)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC015641
• Gene Symbol: ENO1
• Gene ID (NCBI): 2023
• ENSEMBL Gene ID: ENSG00000074800
• RRID: AB_2882482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• UNIPROT ID: P06733
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656)

Publications

Species	Application	Title
human	WB	Tissue Cell; Activation of mTOR/HK2 signaling mitigates effects of PYCR2 depletion in colorectal cells; Authors - Li Chen