ENO1 Monoclonal antibody

ENO1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D6E8

Cat no : 67187-1-Ig

Synonyms

Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH

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Validation Data Gallery

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  WB analysis using 67187-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67187-1-Ig (ENO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67187-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENO1 antibody (67187-1-Ig, Clone: 1D6E8 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, PC-3 cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

67187-1-Ig targets ENO1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: ENO1 fusion protein Ag17518
• Full Name: enolase 1, (alpha)
• Calculated molecular weight: 47 kDa
• Observed molecular weight: 47 kDa
• GenBank accession number: BC015641
• Gene symbol: ENO1
• Gene ID (NCBI): 2023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656)