CoraLite®594-conjugated ENO1 Monoclonal antibody

ENO1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat, Pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D6E8

Cat no : CL594-67187

Synonyms

Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH

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Validation Data Gallery

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  IF Staining of HeLa using CL594-67187

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated ENO1 antibody (CL594-67187, Clone: 1D6E8 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HeLa cells

For other applications, we recommend the unconjugated version of this antibody, 67187-1-Ig

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL594-67187 targets ENO1 in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: ENO1 fusion protein Ag17518
• Full Name: enolase 1, (alpha)
• Calculated molecular weight: 47 kDa
• Observed molecular weight: 47 kDa
• GenBank accession number: BC015641
• Gene symbol: ENO1
• Gene ID (NCBI): 2023
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 593 nm / 614 nm
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. It is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. ENO1 may also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. It stimulates immunoglobulin production. (PMID:2005901).