ENTPD7 Polyclonal antibody

ENTPD7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24558-1-AP

Synonyms

Lysosomal apyrase-like protein 1, LALP1, EC:3.6.1.15

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Validation Data Gallery

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  WB analysis of HeLa using 24558-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24558-1-AP (ENTPD7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 24558-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24558-1-AP (ENTPD7 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 24558-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24558-1-AP (ENTPD7 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 24558-1-AP

  Immunofluorescent analysis of HeLa cells using 24558-1-AP (ENTPD7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24558-1-AP targets ENTPD7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENTPD7 fusion protein Ag18417
• Full Name: ectonucleoside triphosphate diphosphohydrolase 7
• Calculated Molecular Weight: 604 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC122857
• Gene Symbol: ENTPD7
• Gene ID (NCBI): 57089
• RRID: AB_2879607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ectonucleoside triphosphate diphosphohydrolase 7 (ENTPD7), also known as LALP1, is a member of the ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) family. It is located in the nucleoplasm and has an effect on oxidative stress, DNA damage and ageing (PMID: 27737960).