CoraLite® Plus 488-conjugated EPHA7 Monoclonal antibody

EPHA7 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A7E5

Cat no : CL488-66667

Synonyms

EHK 3, EHK3, EK11, EPH homology kinase 3, EPH like kinase 11, EPH receptor A7, EPHA7, Ephrin type A receptor 7, HEK11

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Validation Data Gallery

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  FC experiment of SH-SY5Y using CL488-66667

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EPHA7 (CL488-66667, Clone:1A7E5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66667 targets EPHA7 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPHA7 fusion protein Ag3813
• Full Name: EPH receptor A7
• Calculated Molecular Weight: 998 aa, 112 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC027940
• Gene Symbol: EPHA7
• Gene ID (NCBI): 2045
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ephrin receptor A7 (EphA7) is a member of the Eph receptor family. EPHA7 plays a role in corticogenesis processes, determines brain size and shape, and is involved in development of the central nervous system (PMID:34176129). In addition, EphA7 also has a dual role of tumor promoter and tumor suppressor. It can participate in cell proliferation, migration and apoptosis through various mechanisms, and afect tumor diferentiation, staging and prognosis. EphA7 may be a potential diagnostic marker and tumor treatment target (PMID:35112312).