EPHB1 Monoclonal antibody

EPHB1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B7C12

Cat no : 67080-1-Ig

Synonyms

EK6, ELK, EPH like kinase 6, EPH receptor B1, EPHB1, Ephrin type B receptor 1, EPHT2, FLJ37986, Hek6, NET

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Validation Data Gallery

• 

  WB analysis of mouse cerebellum using 67080-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 67080-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67080-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, mouse brain tissue, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67080-1-Ig targets EPHB1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPHB1 fusion protein Ag16347
• Full Name: EPH receptor B1
• Calculated Molecular Weight: 984 aa, 110 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC111744
• Gene Symbol: EPHB1
• Gene ID (NCBI): 2047
• RRID: AB_2882388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPHB1, also named as EPHT2, NET, HEK6 and ELK, belongs to the protein kinase superfamily, Tyr protein kinase family, and Ephrin receptor subfamily. It is a receptor for members of the ephrin-B family. EPHB1 binds to ephrin-B1, -B2 and -B3. EPHB1 may be involved in cell-cell interactions in the nervous system. EPHB1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Publications

Species	Application	Title
rat	WB	Mol Vis; EphB1 causes retinal damage through inflammatory pathways in the retina and retinal Müller cells; Authors - Li Liu