HER2/ErbB2 Monoclonal antibody

HER2/ErbB2 Monoclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1B12A7

Cat no : 60311-1-Ig

Synonyms

CD340, ERBB2, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1



Tested Applications

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 2 publications below

Product Information

60311-1-Ig targets HER2/ErbB2 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HER2/ErbB2 fusion protein Ag16463
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Calculated molecular weight 1255 aa, 138 kDa
GenBank accession numberBC156755
Gene symbol ERBB2
Gene ID (NCBI) 2064
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.

Protocols

Product Specific Protocols
IHC protocol for HER2/ErbB2 antibody 60311-1-IgDownload protocol
IF protocol for HER2/ErbB2 antibody 60311-1-IgDownload protocol
FC protocol for HER2/ErbB2 antibody 60311-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

Circulating HER-2 mRNA in the peripheral blood as a potential diagnostic and prognostic biomarker in females with breast cancer.

Authors - Yanlin Wu
humanIHC

J Cancer

InvivoPen: A novel plasma source for in vivo cancer treatment.

Authors - Xin Zhou