CoraLite®488-conjugated HER2/ErbB2 Monoclonal antibody

HER2/ErbB2 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1B12A7

Cat no : CL488-60311

Synonyms

CD340, ERBB2, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1



Tested Applications

Positive IF detected inhuman breast cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 60311-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60311 targets HER2/ErbB2 in IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HER2/ErbB2 fusion protein Ag16463
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Calculated molecular weight 1255 aa, 138 kDa
GenBank accession numberBC156755
Gene symbol ERBB2
Gene ID (NCBI) 2064
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated HER2/ErbB2 antibody CL488-60311Download protocol
Standard Protocols
Click here to view our Standard Protocols