HER2/ErbB2 Monoclonal antibody, PBS Only

HER2/ErbB2 Monoclonal Antibody for IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B12A7

Cat no : 60311-1-PBS

Synonyms

CD340, ERBB2, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1

---

Validation Data Gallery

• 

  IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SK-BR-3 using 60311-1-Ig (same clone as 60311-1-PBS)

  1X10^6 SK-BR-3 cells were intracellularly stained with 0.4 ug Anti-Human HER2/ErbB2 (60311-1-Ig, Clone:1B12A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60311-1-PBS targets HER2/ErbB2 in IF, FC, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HER2/ErbB2 fusion protein Ag16463
• Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Calculated Molecular Weight: 1255 aa, 138 kDa
• GenBank Accession Number: BC156755
• Gene Symbol: ERBB2
• Gene ID (NCBI): 2064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.