ERLIN1 Recombinant antibody

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  WB analysis using 85377-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85377-1-RR (ERLIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ERLIN1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85377-1-RR

Clone No.242778B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Erlin-1, Erlin 1, ER lipid raft associated 1, Endoplasmic reticulum lipid raft-associated protein 1, C10orf69

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Tested Applications

• Positive WB detected in: U-937 cells, HL-60 cells, HepG2 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85377-1-RR targets ERLIN1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ERLIN1 fusion protein Ag11393
• Full Name: ER lipid raft associated 1
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC031791
• Gene Symbol: ERLIN1
• Gene ID (NCBI): 10613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75477
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERLIN1, also known as Erlin-1 or SPFH1, stands for "ER lipid raft-associated protein 1." It is a protein-coding gene that plays a significant role in cellular processes, particularly in the endoplasmic reticulum (ER). The ER is a crucial organelle involved in protein synthesis, lipid metabolism, and calcium homeostasis. ERLIN1 is part of the SPFH (stomatin, prohibitin, flotillin, and HflK/C) domain-containing family of proteins, which are often associated with lipid rafts-microdomains within cellular membranes that are enriched in cholesterol and sphingolipids. ERLIN1 is involved in the regulation of protein degradation, particularly through the ER-associated degradation (ERAD) pathway (PMID: 37683630).