ERLIN1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85377-1-RR (same clone as 85377-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85377-1-RR (ERLIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85377-1-PBS in a different storage buffer formulation.

ERLIN1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85377-1-PBS

Clone No.242778B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

242778B10, C10orf69, Endoplasmic reticulum lipid raft-associated protein 1, ER lipid raft associated 1, Erlin 1

---

Product Information

85377-1-PBS targets ERLIN1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11393

  Product name: Recombinant human ERLIN1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 29-346 aa of BC031791

  Sequence: GHLAVYYRGGALLTSPSGPGYHIMLPFITTFRSVQTTLQTDEVKNVPCGTSGGVMIYIDRIEVVNMLAPYAVFDIVRNYTADYDKTLIFNKIHHELNQFCSAHTLQEVYIELFDQIDENLKQALQKDLNLMAPGLTIQAVRVTKPKIPEAIRRNFELMEAEKTKLLIAAQKQKVVEKEAETERKKAVIEAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLAREKAKADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSSCALKYSDIRTGRESSLPSKEALEPSGENVIQNKESTG

• Full Name: ER lipid raft associated 1
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC031791
• Gene Symbol: ERLIN1
• Gene ID (NCBI): 10613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75477
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ERLIN1, also known as Erlin-1 or SPFH1, stands for "ER lipid raft-associated protein 1." It is a protein-coding gene that plays a significant role in cellular processes, particularly in the endoplasmic reticulum (ER). The ER is a crucial organelle involved in protein synthesis, lipid metabolism, and calcium homeostasis. ERLIN1 is part of the SPFH (stomatin, prohibitin, flotillin, and HflK/C) domain-containing family of proteins, which are often associated with lipid rafts-microdomains within cellular membranes that are enriched in cholesterol and sphingolipids. ERLIN1 is involved in the regulation of protein degradation, particularly through the ER-associated degradation (ERAD) pathway (PMID: 37683630).