EXOC3L2 Monoclonal antibody

EXOC3L2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3C1C2

Cat no : 66537-1-Ig

Synonyms

EXOC3L2, XTP7

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Validation Data Gallery

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  WB analysis of HUVEC using 66537-1-Ig

  HUVEC, HEK-293 and U-251 cells were subjected to SDS PAGE followed by western blot with 66537-1-Ig (EXOC3L2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HUVEC using 66537-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using EXOC3L2 antibody (66537-1-Ig, Clone: 3C1C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HUVEC cells
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1500-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66537-1-Ig targets EXOC3L2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EXOC3L2 fusion protein Ag26912
• Full Name: exocyst complex component 3-like 2
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC104947
• Gene Symbol: EXOC3L2
• Gene ID (NCBI): 90332
• RRID: AB_2881900
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The exocyst is a protein complex that ensures spatial targeting of exocytotic vesicles to the plasma membrane. Exocyst complex component 3-like 2 (EXOC3L2) associates with the exocyst complex and mediates directional migration of endothelial cells (PMID: 21566143). The gene of human EXOC3L2 maps to chromosome 19q13.32 and encodes a deduced 409-amino acid protein with a calculated molecular mass of about 46 kDa. Mouse Exoc3l2 gene encodes an approximately 250-amino acid protein that lacks the N-terminal domain of human EXOC3L2. Mutations in EXOC3L2 gene may be associated with Alzheimer's disease.

Publications

Species	Application	Title
human	WB	J Med Genet; Biallelic mutations in EXOC3L2 cause a novel syndrome that affects the brain, kidney and blood.; Authors - Adel Shalata