EXOC3L2 Monoclonal antibody, PBS Only

• 

  WB analysis of HUVEC using 66537-1-Ig (same clone as 66537-1-PBS)

  HUVEC, HEK-293 and U-251 cells were subjected to SDS PAGE followed by western blot with 66537-1-Ig (EXOC3L2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66537-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 66537-1-Ig (same clone as 66537-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using EXOC3L2 antibody (66537-1-Ig, Clone: 3C1C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66537-1-PBS in a different storage buffer formulation.

EXOC3L2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 66537-1-PBS

Clone No.3C1C2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EXOC3L2, XTP7

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Product Information

66537-1-PBS targets EXOC3L2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26912

  Product name: Recombinant human EXOC3L2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 16-278 aa of BC104947

  Sequence: GTLRGLEDECVTDVKAQTRAALLRVLQEDEEHWGSLEDQPSSLAQDVCELLEEHTERAPRISQEFGERMAHCCLGGLAEFLQSFQQRVERFHENPAVREMLPDTYISKTIALVNCGPPLRALAERLARVGPPESEPAREASASALDHVTRLCHRVVANLLFQELQPHFNKLMRRKWLSSPEALDGIVGTLGAQALALRRMQDEPYQALVAELHRRALVEYVRPLLRGRLRCSSARTRSRVAGRLREDAAQLQRLFRRLESQAS

• Full Name: exocyst complex component 3-like 2
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC104947
• Gene Symbol: EXOC3L2
• Gene ID (NCBI): 90332
• RRID: AB_2881900
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q2M3D2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The exocyst is a protein complex that ensures spatial targeting of exocytotic vesicles to the plasma membrane. Exocyst complex component 3-like 2 (EXOC3L2) associates with the exocyst complex and mediates directional migration of endothelial cells (PMID: 21566143). The gene of human EXOC3L2 maps to chromosome 19q13.32 and encodes a deduced 409-amino acid protein with a calculated molecular mass of about 46 kDa. Mouse Exoc3l2 gene encodes an approximately 250-amino acid protein that lacks the N-terminal domain of human EXOC3L2. Mutations in EXOC3L2 gene may be associated with Alzheimer's disease.