FAM3A Polyclonal antibody

FAM3A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20588-1-AP

Synonyms

2 19, 2.19, Cytokine like protein 2 19, DXS560S, FAM3A, Protein FAM3A, XAP 7

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Validation Data Gallery

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  WB analysis using 20588-1-AP

  Mouse liver tissues were subjected to SDS PAGE followed by western blot with 20588-1-AP (FAM3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 20588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20588-1-AP (FAM3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 20588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20588-1-AP (FAM3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

20588-1-AP targets FAM3A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM3A fusion protein Ag14343
• Full Name: family with sequence similarity 3, member A
• Calculated Molecular Weight: 230 aa, 25 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: BC002934
• Gene Symbol: FAM3A
• Gene ID (NCBI): 60343
• RRID: AB_2878704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Neural Regen Res; The circular RNA Rap1b promotes Hoxa5 transcription by recruiting Kat7 and leading to increased Fam3a expression, which inhibits neuronal apoptosis in acute ischemic stroke; Authors - Fang-Fang Zhang
human	WB	In Vitro Cell Dev Biol Anim; FAM3A mediates the phenotypic switch of human aortic smooth muscle cells stimulated with oxidised low-density lipoprotein by influencing the PI3K-AKT pathway; Authors - Lei Yang ; KD Validated