|Positive WB detected in||HepG2 cells, HEK-293 cells, Jurkat cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
66591-1-Ig targets FASN in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||FASN fusion protein Ag0975|
|Full Name||fatty acid synthase|
|Calculated molecular weight||272 kDa|
|Observed molecular weight||272 kDa|
|GenBank accession number||BC007909|
|Gene ID (NCBI)||2194|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.
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