FASN Monoclonal antibody
FASN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (3)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1B6E4
Cat no : 66591-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HEK-293 cells, Jurkat cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 11 publications below |
IHC | See 3 publications below |
IF | See 2 publications below |
IP | See 2 publications below |
Product Information
66591-1-Ig targets FASN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse, monkey |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | FASN fusion protein Ag0975 |
Full Name | fatty acid synthase |
Calculated Molecular Weight | 272 kDa |
Observed Molecular Weight | 272 kDa |
GenBank Accession Number | BC007909 |
Gene Symbol | FASN |
Gene ID (NCBI) | 2194 |
RRID | AB_2881951 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.
Protocols
Product Specific Protocols | |
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WB protocol for FASN antibody 66591-1-Ig | Download protocol |
IHC protocol for FASN antibody 66591-1-Ig | Download protocol |
IF protocol for FASN antibody 66591-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. | ||
Theranostics Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription. | ||
J Transl Med TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis | ||
Am J Cancer Res Ubiquitin specific peptidase 38 promotes the progression of gastric cancer through upregulation of fatty acid synthase. | ||
Environ Toxicol SIRT1/mTOR pathway-mediated autophagy dysregulation promotes Pb-induced hepatic lipid accumulation in HepG2 cells |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sun (Verified Customer) (01-05-2023) | We detected a band around 200-300kDa region in HEK293T cells using this antibody.
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