FcZero-rAb™ PE Anti-Human FLT3/CD135 Rabbit Recombinant Antibody

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  FC experiment of Reh using PE-FcA98106

  1x10^6 Reh cells (left) or U-937 cells (right) were surface stained with 5 ul PE Anti-Human FLT3/CD135 Rabbit Recombinant Antibody (PE-FcA98106, Clone: 241414F8) (red), or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

FLT3/CD135 FcZero-rAb^TM Recombinant Antibody for FC

Cat No. PE-FcA98106

Clone No.241414F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

FLT3, FLK2, FL cytokine receptor, Fetal liver kinase-2, EC:2.7.10.1

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Tested Applications

• Positive FC detected in: Reh cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98106 targets FLT3/CD135 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: fms-related tyrosine kinase 3
• Calculated Molecular Weight: 993 aa, 113 kDa
• GenBank Accession Number: BC036028
• Gene Symbol: FLT3
• Gene ID (NCBI): 2322
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.