FPGT Polyclonal antibody

FPGT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24937-1-AP

Synonyms

FPGT, GDP L fucose diphosphorylase, GDP L fucose pyrophosphorylase, GFPP

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Validation Data Gallery

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  WB analysis of mouse colon using 24937-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 24937-1-AP (FPGT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 24937-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 24937-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 24937-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 24937-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse colon tissue
• Positive IHC detected in: human colon tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24937-1-AP is FPGT Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FPGT fusion protein Ag20655
• Full Name: fucose-1-phosphate guanylyltransferase
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC032308
• Gene Symbol: FPGT
• Gene ID (NCBI): 8790
• RRID: AB_2879808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FPGT(Fucose-1-phosphate guanylyltransferase) is also named as GFPP. This protein catalyzes the formation of GDP-L-fucose from GTP and L-fucose-1-phosphate. It also functions as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. FPGT has 6 isoforms produced by alternative splicing.