FRZB Monoclonal antibody

FRZB Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2B4A1

Cat no : 67081-1-Ig

Synonyms

FIZ, FRE, Frezzled, FRITZ, frizzled related protein, Frizzled related protein 1, FRP, FRP 3, FRZB, FRZB 1, FRZB PEN, FRZB1, FZRB, hFIZ, sFRP 3, SFRP3, SRFP3

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Validation Data Gallery

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  WB analysis using 67081-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67081-1-Ig (FRZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67081-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67081-1-Ig (FRZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, 4T1 cells, MKN-45 cells, SGC-7901 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67081-1-Ig targets FRZB in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FRZB fusion protein Ag28147
• Full Name: frizzled-related protein
• Calculated Molecular Weight: 325 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC027855
• Gene Symbol: FRZB
• Gene ID (NCBI): 2487
• RRID: AB_2882389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Frzb (also known as sFRP3) is a member of the secreted frizzled related protein (SFRP) family, originally identified as a chondrogenic factor during bone morphogenesis (PMID: 29209231). It was subsequently shown to be a regulator in Wnt signaling, and also have a role in regulating cell growth and differentiation in specific cell types. FRZB was seen to be acting as an oncogene in some cancer, such as metastatic renal cancer.

Publications

Species	Application	Title
human	WB	Eur J Pharmacol; Eltrombopag restores proliferative capacity and adipose-osteogenic balance of mesenchymal stromal cells in low-risk myelodysplastic syndromes; Authors - Minghua Hong
human	WB	Stem Cells Dev; Chlorogenic Acid Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Wnt Signaling.; Authors - Xiaoping Hu