• Featured Product
  • KD/KO Validated

FTO Polyclonal antibody

FTO Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IP, IF, RIP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 27226-1-AP

Synonyms

FTO, KIAA1752



Tested Applications

Positive WB detected inA549 cells, Caco-2 cells, HEK-293 cells, HeLa cells, HEK-293T cells, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse testis tissue, human liver cancer tissue, mouse brain tissue, human colon tissue, human stomach cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman lymphoma tissue, human liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27226-1-AP targets FTO in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, goat, chicken, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FTO fusion protein Ag26095
Full Name fat mass and obesity associated
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_001080432
Gene Symbol FTO
Gene ID (NCBI) 79068
RRIDAB_2880809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

Protocols

Product Specific Protocols
WB protocol for FTO antibody 27226-1-APDownload protocol
IHC protocol for FTO antibody 27226-1-APDownload protocol
IF protocol for FTO antibody 27226-1-APDownload protocol
IP protocol for FTO antibody 27226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A druggable copper-signalling pathway that drives inflammation

Authors - Stéphanie Solier
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li
  • KO Validated
mouseWB,RIP

Nat Cell Biol

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.

Authors - Xiang-Qian Gao
human,mouseWB,IP

Nat Commun

Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma

Authors - Weiwei Dai
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
humanWB

Adv Sci (Weinh)

RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation

Authors - Yu Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xiaochen (Verified Customer) (07-08-2024)

Show good sepecific image in WB without non-specific bend.

  • Applications: Western Blot
  • Cell Tissue Type: U2OS
FTO Antibody Western Blot validation ( dilution) in U2OS (Cat no:27226-1-AP)
FH

Katherine (Verified Customer) (09-25-2023)

Quality was good.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

S (Verified Customer) (03-20-2023)

Very Good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: AC16
FTO Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:27226-1-AP)
FH

SU (Verified Customer) (03-22-2022)

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
FTO Antibody Western Blot validation ( dilution) in H9C2 (Cat no:27226-1-AP)
FH

P. (Verified Customer) (05-15-2021)

Excellent antibody!

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
FTO Antibody Western Blot validation ( dilution) in H9C2 (Cat no:27226-1-AP)