Validation Data Gallery
|Positive WB detected in||A549 cells, Caco-2 cells, HEK-293 cells, HeLa cells, HEK-293T cells, SH-SY5Y cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse testis tissue, mouse brain tissue, human colon tissue, human stomach cancer tissue, human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human lymphoma tissue|
|Western Blot (WB)||WB : 1:1000-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 27226-1-AP is FTO Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FTO fusion protein Ag26095|
|Full Name||fat mass and obesity associated|
|Calculated molecular weight||58 kDa|
|Observed molecular weight||58 kDa|
|GenBank accession number||NM_001080432|
|Gene ID (NCBI)||79068|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.
|Product Specific Protocols|
|WB protocol for FTO antibody 27226-1-AP||Download protocol|
|IHC protocol for FTO antibody 27226-1-AP||Download protocol|
|IF protocol for FTO antibody 27226-1-AP||Download protocol|
|IP protocol for FTO antibody 27226-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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