FTO Polyclonal antibody

• Featured Product
• KD/KO Validated

FTO Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

165

Reviews (5)

Cat no : 27226-1-AP

Synonyms

KIAA1752

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Validation Data Gallery

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  WB analysis using 27226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27226-1-AP (FTO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27226-1-AP (FTO antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lymphoma using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lymphoma using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 27226-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (27226-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, Caco-2 cells, HEK-293 cells, HeLa cells, HEK-293T cells, SH-SY5Y cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human colon tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human lymphoma tissue, human liver cancer tissue
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 23 publications below
• WB: See 151 publications below
• IHC: See 34 publications below
• IF: See 16 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below
• RIP: See 13 publications below

Product Information

27226-1-AP targets FTO in WB, IHC, IF-P, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, chicken, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FTO fusion protein Ag26095
• Full Name: fat mass and obesity associated
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_001080432
• Gene Symbol: FTO
• Gene ID (NCBI): 79068
• RRID: AB_2880809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

Publications

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human	WB	Nat Genet; N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.; Authors - Yuan Li ; KO Validated
mouse	WB,RIP	Nat Cell Biol; The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.; Authors - Xiang-Qian Gao
human,mouse	WB,IP	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
mouse	WB	Adv Sci (Weinh); PXR Activation Relieves Deoxynivalenol-Induced Liver Oxidative Stress Via Malat1 LncRNA m6A Demethylation; Authors - Yue Feng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaochen (Verified Customer) (07-08-2024)

Show good sepecific image in WB without non-specific bend.

• Applications: Western Blot
• Cell Tissue Type: U2OS

FTO Antibody Western Blot validation ( dilution) in U2OS (Cat no:27226-1-AP)

FH

Katherine (Verified Customer) (09-25-2023)

Quality was good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

S (Verified Customer) (03-20-2023)

Very Good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: AC16

FTO Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:27226-1-AP)

FH

SU (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: H9C2

FTO Antibody Western Blot validation ( dilution) in H9C2 (Cat no:27226-1-AP)

FH

P. (Verified Customer) (05-15-2021)

Excellent antibody!

• Applications: Western Blot
• Cell Tissue Type: H9C2

FTO Antibody Western Blot validation ( dilution) in H9C2 (Cat no:27226-1-AP)