• Featured Product
  • KD/KO Validated

FTO Polyclonal antibody

FTO Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27226-1-AP

Synonyms

FTO, KIAA1752



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, mouse brain tissue, HeLa cells, Caco-2 cells
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27226-1-AP is FTO Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FTO fusion protein Ag26095
Full Name fat mass and obesity associated
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_001080432
Gene symbol FTO
Gene ID (NCBI) 79068
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

Protocols

Product Specific Protocols
WB protocol for FTO antibody 27226-1-APDownload protocol
IHC protocol for FTO antibody 27226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Epigenomics

Alteration of N6-methyladenosine epitranscriptome profile in unilateral ureteral obstructive nephropathy.

Authors - Xueyan Li
mouseWB

Animals (Basel)

Resveratrol Attenuates Aflatoxin B1-Induced ROS Formation and Increase of m6A RNA Methylation.

Authors - Jiamin Wu
humanWB

Cell Death Dis

METTL14-regulated PI3K/Akt signaling pathway via PTEN affects HDAC5-mediated epithelial-mesenchymal transition of renal tubular cells in diabetic kidney disease.

Authors - Zhaoxia Xu
humanWB,IHC

Pathol Res Pract

FTO accelerates ovarian cancer cell growth by promoting proliferation, inhibiting apoptosis, and activating autophagy.

Authors - Lin Zhao
  • KD Validated
mouseWB,IHC

Arch Gynecol Obstet

Decreased expression of m6A demethylase FTO in ovarian aging.

Authors - Xiaoyan Sun
mouse,ratWB

Mol Cell Biochem

Increased m6A-RNA methylation and FTO suppression is associated with myocardial inflammation and dysfunction during endotoxemia in mice.

Authors - Praveen K Dubey

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

P. (Verified Customer) (05-15-2021)

Excellent antibody!

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
FTO Antibody Western Blot validation ( dilution) in H9C2 (Cat no:27226-1-AP)