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FTO Polyclonal antibody
FTO Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 27226-1-AP
Validation Data Gallery
|Positive WB detected in
|A549 cells, Caco-2 cells, HEK-293 cells, HeLa cells, HEK-293T cells, SH-SY5Y cells
|Positive IP detected in
|mouse brain tissue
|Positive IHC detected in
|mouse testis tissue, human liver cancer tissue, mouse brain tissue, human colon tissue, human stomach cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|human lymphoma tissue, human liver cancer tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:3000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
27226-1-AP targets FTO in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat, chicken
|Host / Isotype
|Rabbit / IgG
|FTO fusion protein Ag26095
|fat mass and obesity associated
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.
|Product Specific Protocols
|WB protocol for FTO antibody 27226-1-AP
|IHC protocol for FTO antibody 27226-1-AP
|IF protocol for FTO antibody 27226-1-AP
|IP protocol for FTO antibody 27226-1-AP
|Click here to view our Standard Protocols
A druggable copper-signalling pathway that drives inflammation
N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.
Nat Cell Biol
The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.
mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.
Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.
PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Katherine (Verified Customer) (09-25-2023)
Quality was good.
S (Verified Customer) (03-20-2023)
SU (Verified Customer) (03-22-2022)
P. (Verified Customer) (05-15-2021)