HRP-conjugated DYKDDDDK Tag Monoclonal antibody (Binds to FLAG® tag epitope)
DYKDDDDK Tag Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2b
Reactivity
recombinant protein
Applications
WB
Conjugate
HRP Fluorescent Dye
CloneNo.
1E7B4
Cat no : HRP-66008
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Recombinant protein |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
Product Information
HRP-66008 targets DYKDDDDK Tag in WB applications and shows reactivity with recombinant protein samples.
Background Information
DYKDDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDYKDDDDK. The structure of DYKDDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DYKDDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated DYKDDDDK Tag antibody. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Publications
Species | Application | Title |
---|---|---|
Biochem Biophys Res Commun Vaccination with a DNA vaccine encoding CD317-targeting HBs antigen elicits enhanced immunity in mice. | ||
Nat Commun FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease. |