Tested Applications
| Positive WB detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-66008 targets DYKDDDDK Tag in WB, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | DYKDDDDK Tag |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2883835 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DYKDDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDYKDDDDK. The structure of DYKDDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DYKDDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated DYKDDDDK Tag antibody. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP DYKDDDDK Tag antibody HRP-66008 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
HRP-conjugated DYKDDDDK Tag Monoclonal antibody (Binds to FLAG® tag epitope), catalog number HRP-66008, has 54 citations. Journals include Cell, Nature Communications, Molecular Cancer, Gastroenterology, Annals of the Rheumatic Diseases, Cell Reports, EMBO Reports, Development, Plant Cell, and others. Research fields span cancer biology, immunology, virology, neuroscience, plant biology, metabolism, cell signaling, and infectious disease.
| Species | Application | Title |
|---|---|---|
Mol Cancer Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity | ||
Gastroenterology BMPR2 as a novel predisposition gene for hereditary colorectal polyposis | ||
Ann Rheum Dis Lupus and inflammatory bowel disease share a common set of microbiome features distinct from other autoimmune disorders | ||
Ann Rheum Dis Lupus and inflammatory bowel disease share a common set of microbiome features distinct from other autoimmune disorders | ||
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot. One user reported specific detection of a GFP-3xFLAG tag protein in HEK293T cells at a 1:10,000 dilution. The other, using a 1:5,000 dilution across HEK293T, SKOV3, and A2780 cells, praised its excellent specificity and noted that it significantly facilitated the WB process. Overall, customers highlight strong specificity, ease of use, and reliable performance across multiple cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tsimafei (Verified Customer) (07-13-2026) | GFP-3xFLAG tag protein was specifically detected in HEK293T cells
 |
Chunxu (Verified Customer) (01-31-2025) | Significant facility the process of WB and this antibody results great specifity
|
