CoraLite®594-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope)
DYKDDDDK Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
CoraLite®594 Fluorescent Dye
Cat no : CL594-66008
|Positive IF detected in
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
CL594-66008 targets DYKDDDDK in IF applications and shows reactivity with Recombinant protein samples.
|Host / Isotype
|Mouse / IgG2b
|Gene ID (NCBI)
|CoraLite®594 Fluorescent Dye
|Excitation/Emission maxima wavelengths
|588 nm / 604 nm
|Protein A purification
|PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
|Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.