CoraLite®594-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Recombinant protein

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E7B4

Cat no : CL594-66008

Synonyms

DYKDDDDK, Flag tag



Tested Applications

Positive IF detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66008-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66008 targets DYKDDDDK in IF applications and shows reactivity with Recombinant protein samples.

Tested Reactivity Recombinant protein
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name DYKDDDDK
Gene symbol
Gene ID (NCBI)
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protocols

Product Specific Protocols
IF protocol for CL594 DYKDDDDK antibody CL594-66008Download protocol
Standard Protocols
Click here to view our Standard Protocols