CoraLite®594-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

8H6A10

Cat no : CL594-66008

Synonyms

Flag tag, DYKDDDDK tag, DDDDK tag

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Validation Data Gallery

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  IF Staining of Transfected HEK-293 using CL594-66008

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite®594 DYKDDDDK antibody (CL594-66008, Clone: 8H6A10 ) at dilution of 1:200.

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  FC experiment of Transfected HEK-293T using CL594-66008

  1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope) (CL594-66008, Clone:8H6A10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: Transfected HEK-293 cells
• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66008 targets DYKDDDDK in IF/ICC applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: DYKDDDDK
• RRID: AB_2883474
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.