Tested Applications
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66008 targets DYKDDDDK tag in IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | DYKDDDDK tag |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2883474 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 DYKDDDDK tag antibody CL594-66008 | Download protocol |
| IF protocol for CL594 DYKDDDDK tag antibody CL594-66008 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CoraLite®594-conjugated DYKDDDDK tag Monoclonal antibody (CL594-66008) has 5 citations published in Nature Communications (×2), Oncogenesis, PLoS Pathogens, and Virus Genes. Research fields span cardiac mitochondrial respiration, GPCR–arrestin signaling, breast cancer calcium dynamics, viral–lysosome interactions, and influenza host-protein screening. Applications include immunofluorescence and structural studies.
| Species | Application | Title |
|---|---|---|
Nat Commun Core conformation of arrestin coupling to parathyroid hormone type 1 receptor. | ||
Nat Commun The transcriptional repressor HEY2 regulates mitochondrial oxidative respiration to maintain cardiac homeostasis | ||
Oncogenesis Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth | ||
Virus Genes Screening for influenza B virus NS1-interacting host proteins and characterization of interactions with hnRNPA0 and DDX39B. |
Reviews
What customers say
The single review for CL594-66008 is a 5-star rating from an immunofluorescence application on U2OS cells at a 1:200 dilution. The reviewer praised the antibody's excellent performance, noting it delivers a clear, specific signal with very low background. They described the staining as both reliable and bright, indicating high satisfaction with the product's specificity and sensitivity in IF experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Emile (Verified Customer) (02-20-2026) | Excellent performance in immunofluorescence. The Anti-FLAG Coralite 549 antibody gives a clear, specific signal with very low background. Reliable and bright staining.
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