CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

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  IF Staining of Transfected HEK-293 using CL647-66008

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 DYKDDDDK tag antibody (CL647-66008, Clone: 8H6A10 ) at dilution of 1:200.

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  FC experiment of Transfected HEK-293T using CL647-66008

  1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (CL647-66008, Clone:8H6A10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

DYKDDDDK tag Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL647-66008

Clone No.8H6A10

Publications(1)

Reviews (1)

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra)

Synonyms

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK

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Tested Applications

• Positive IF/ICC detected in: Transfected HEK-293 cells
• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

CL647-66008 targets DYKDDDDK tag in WB, IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: DYKDDDDK tag
• RRID: AB_2920272
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: FLAGTAG
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Adv Sci (Weinh); FUS Selectively Facilitates circRNAs Packing into Small Extracellular Vesicles within Hypoxia Neuron; Authors - Jiankun Zang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rushita (Verified Customer) (10-07-2025)

This antibody is a game-changer! Sensitive and spot on!

• Applications: Immunofluorescence
• Cell Tissue Type: Heart, Kindey, Liver