DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)
DYKDDDDK tag Monoclonal Antibody for IF, IP, WB, ELISA
Cat no : 66008-4-Ig
"DYKDDDDK tag Antibodies" Comparison
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|Positive WB detected in||Transfected HEK-293 cells|
|Positive IP detected in||Transfected HEK-293 cells|
|Positive IF detected in||Transfected HEK-293 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB|
|Immunofluorescence (IF)||IF : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
66008-4-Ig targets DYKDDDDK tag in WB, IP, IF, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
|Tested Reactivity||recombinant protein|
|Cited Reactivity||human, bovine|
|Host / Isotype||Mouse / IgG2b|
|Full Name||DYKDDDDK tag|
|Gene ID (NCBI)|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chun (Verified Customer) (06-19-2022)
Céline (Verified Customer) (03-29-2022)
I have compared with anti-Flag M2 from Sigma in an IP experiment and I found the M2 antibody better.