DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein and More (2)

Applications

WB, IP, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

CloneNo.

8H6A10

Cat no : 66008-4-Ig

Synonyms

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag, Flag tag


"DYKDDDDK tag Antibodies" Comparison

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Applications:
  • WB analysis using 66008-4-Ig

    WB analysis using 66008-4-Ig

    HEK-293 and different loading amount of DYKDDDDK tagged TDP-43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control.

  • WB analysis using 66008-4-Ig

    WB analysis using 66008-4-Ig

    HEK-293 and DYKDDDDK-TDP43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading

  • WB analysis using 66008-4-Ig

    WB analysis using 66008-4-Ig

    HEK-293 and DYKDDDDK-PCSK9 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDKtag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control.

  • IP experiment using 66008-4-Ig

    IP experiment using 66008-4-Ig

    IP result of anti-DYKDDDDK tag(IP:66008-4-Ig, 5 ug; Detection:80010-1-RR 1:10000) with Transfected HEK-293T cells lysate 250 ug.

  • IF Staining of Transfected HEK-293 using 66008-4-Ig

    IF Staining of Transfected HEK-293 using 66008-4-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed DYKDDDDK-TDP43 Transfected HEK-293 cells using DYKDDDDK tag antibody (66008-4-Ig, Clone: 8H6A10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).


Tested Applications

Positive WB detected inTransfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunofluorescence (IF)IF : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66008-4-Ig targets DYKDDDDK tag in WB, IP, IF, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivityhuman, bovine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name DYKDDDDK tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protocols

Product Specific Protocols
WB protocol for DYKDDDDK tag antibody 66008-4-IgDownload protocol
IF protocol for DYKDDDDK tag antibody 66008-4-IgDownload protocol
IP protocol for DYKDDDDK tag antibody 66008-4-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IP

Cell Death Dis

USP26 promotes anaplastic thyroid cancer progression by stabilizing TAZ.

Authors - Jianing Tang
humanWB,IF

Oxid Med Cell Longev

Cell-Dependent Pathogenic Roles of Filamin B in Different Skeletal Malformations.

Authors - Huixiao Wu
bovineWB

J Anim Sci

MiR-18 inhibitor promotes the differentiation of bovine skeletal muscle-derived satellite cells by increasing MEF2D expression.

Authors - Bowen Meng
WB

Oral Dis

SATB1 promotes osteogenic differentiation of diabetic rat BMSCs through MAPK signalling activation.

Authors - Jing Guo
CoIP

Poult Sci

Myricetin exerts its antiviral activity against infectious bronchitis virus by inhibiting the deubiquitinating activity of papain-like protease.

Authors - Shuwei Peng
WB

Vet Res

Lawsonia intracellularis LI0666 is a new EPIYA effector exported by the Yersinia enterocolitica type III secretion system.

Authors - Cang Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chun (Verified Customer) (06-19-2022)

This antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
FH

Céline (Verified Customer) (03-29-2022)

I have compared with anti-Flag M2 from Sigma in an IP experiment and I found the M2 antibody better.

  • Applications: Immunoprecipitation
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Hela cells